PROTEASES

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[Protease - pronounced pro-tee-ayse]

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My final year project, supervised by Dr. Catherine Adley, was entitled
"Bioinformatics and Laboratory Analysis of Proteases".

Proteases (also known as proteolytic enzymes, proteinases or peptidases), are enzymes which break down proteins by hydrolysing peptide bonds. Like all enzymes, proteases are biological catalysts which speed up biochemical reactions, without the protease itself being changed in the process.

Some proteases result in the breakdown of a protein into its constituent amino acids while others just break off a segment of the protein chain. Exopeptidases cleave (break off) an amino acid or amino-acid-chain from the end of a protein chain, and endopeptidases cleave amino acids within the protein chain.
Much research is taking place on the function and activity of various different proteases. This page will provide links to many web pages describing this research.

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PROTEASE LINKS:

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GENERAL PROTEASE LINKS

• PROLYSIS A protease and protease inhibitor Web server at the University of Tours, France.
  
• Craik Lab Home Page "Welcome to the World of Proteases" at the University of California, San Francisco.
  
• A Proteolysis page under construction

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HIV Protease Page HIV Proteolytic Enzymes and HIV Protease Inhibitors

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Bacterial Proteases

• HS1VU A new type of ATP-dependent protease in Escherichia coli
  from the Department of Cell Biology at Harvard Medical School
  PNAS : HS1VU in the Proceedings of the National Academy of Sciences.
  
• Structure-function studies of the ATP-dependent Clp protease from E. coli

Protease Inhibitors

• The Complete Guide for Protease Inhibition
  
• Call For Papers on Computational Studies on the design of protease inhibitors.
• Ovoinhibitor A Serine Protease Inhibitor purified from hen egg white.
• Interleukin-1 beta converting enzyme ( ICE ) Inhibitors
• Herpesvirus protease inhibitors.
  
• A conference on Protease Inhibitors

Viral Proteases

Retroviral proteases
Viral Proteases and their role in Virus Assembly

Fungal Proteases

Protozoan Protease


Protease Assays:

• A Sensitive Protease Activity Assay Using Fluorescence Polarization
  

Serine Proteases

• Structure, function, regulation and von Willebrand factor
  
• Serine proteases in general
• Active Site
• Homology Modelling

Acid Proteases

Bacterial Metalloproteases

A Cysteine Protease: Papain, and its relationship to the Serine Proteases

Drosophila melanogaster - Protein Degradation

Saccharomyces cerevisiae - Protein Degradation

Bacillus megaterium - Protein Degradation

Anabaena sp. - Protein degradation

Mechanisms: Nitrogen-15 NMR spectroscopy of the Active-Site Histidine of alpha-Lytic Protease.

A Protease that triggers Apoptosis

A Metalloprotease involved in the Processing of Opioid Neuropeptide Precursors

Proteases in Blood Coagulation

Medline: Loss of protease activity may lead to cataract formation in the aged lens.

Proteases as Digestive Aids

Meat Processing Enzymes

Fibrinolytic Enzymes

Protease Inhibitors and Haemophilia

Protease mRNA Degradation

Neutral Protease from Bacillus

Thermophilic Protease

An NRC Protease for Specific Processing of Secreted proteins in genetically modified yeast cells.

Plant Protease Gene Sequences:

Cloning of a tomato cDNA of a Clp-like Energy Dependent Protease
A carboxyl-terminal processing protease in the cyanobacterium Synechocystis sp.

Structure-Based Design and Two Aspartic Proteases

X-Ray Crystallography Images of Protease

Protein Mass Spectrometry Endoprotease and Exopeptidase protocols for protein mass spectrometry.

Residue-specific Proteases in Protein Fragmentation

Profluorescent protease substrates for enzymatic activity determination in serum as well as whole cells.

University College Santa Cruz WWW Lectures: Serine Proteases, Other Classes of Proteases

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